Date, Place: 3월 15일(목) 오후 2시, 지곡연구동 304호
Speaker: Dr. Sung Jun Lim (DGIST)
Subject: Engineering Fluorescent Semiconductor Nanocrystals for Bioimaging
Colloidal semiconductor nanocrystals (NCs), widely known as quantum dots, are protein-sized
fluorescent particles that have emerged as versatile fluorescent labels in biomolecular imaging. NCs
exhibit many unique optical properties such as size-dependent emission color, narrow emission with
broad absorption, and bright emission with long-term photostablility. Moreover, these light-emitting
particles can be easily biologically functionalized by applying conventional bio-conjugation strategies.
These features have enabled simultaneous imaging of multiple biomolecules and long-term tracking of
single biomolecules, which were unfeasible with traditional fluorescent labels such as molecular dyes and
fluorescent proteins. Furthermore, continuing efforts have been focused on not only to optimize
aforementioned NC properties for their general use in bioimaging but also to engineer new NCs with
unprecedented properties that are potentially suitable for advanced imaging and other biological
In this presentation, I will briefly explain general properties of NCs and their use in bioimaging. And
discuss the current focuses on NC researches to engineer new NCs for advanced bio applications including
developing NCs with equalized single-particle brightnesses for multi-color quantitative imaging , flat
fluorescent NCs (nanoplatelets) showing unusually rapid cellular entry , and bright near-infrared
emitting NCs for highly sensitive single biomolecular imaging.
1. Lim et al., Brightness-Equalized Quantum Dots, Nat. Commun. 2015, 6, 8210.
2. Lim et al., Lipoprotein-Nanoplatelets: Brightly Fluorescent, Zwitterionic Probes with Rapid Cellular
Entry, J. Am. Chem. Soc. 2016, 138, 64.